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发布于:2019-10-9 22:29:48  访问:98 次 回复:0 篇
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Is factor in coordination of the oxidative stress response.ResultsGene ontology
We used SAM to evaluate the significance of variations in expression of each gene [21]. The dose of selenite used was the lowest dose that significantly altered cell survival in our preliminary growth assays (data not shown). This toxic dose of selenite induced large changes in the transcriptome of the cells (see additional file 1). In our data set, about 30 of the yeast ORFs displayed a significant change in expression level for at least two consecutive time points, with similar numbers of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20525876 upand downregulated. The earliest significant effects on gene expression were detected at 10 minutes, with the peakPage 2 of(page number not for citation purposes)BMC Genomics 2008, 9:http://www.biomedcentral.com/1471-2164/9/response at 40 minutes (see additional file 2 and additional file 1). We investigated the functional pathways involved in this large and complex response using the tprofiler software [22], which TPPB CAS identified the Gene Ontology (GO) categories significantly up- or downregulated over the whole dataset (figure 1A, see additional file 3). PF-04447943 site T-profiler analysis indicated that iron homeostasis genes were among the first to be induced (figure 1A). Selenite also induced an oxidative stress response characterized by the Tipranavir Purity & Documentation up-regulation of genes involved in redox homeostasis and in proteasome activity (figure 1A). Finally, our selenite treatment, similarly to all stresses with deleteriousFigure 1 PF-04447943 custom synthesis Global Gene Ontology mapping of the selenite response in S. cerevisiae Global Gene Ontology mapping of the selenite response in S. cerevisiae. (A): The gene ontology categories with levels of expression significantly changed by selenite were identified by T-profiler [22]. The graph represents only the most significant functional categories, as a function of time. More complete T-profiler results are available in additional file 3. The color code is as follows: black: E-value > 0.05 (non significant variation); red: E-value < 0.05 and t-value PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26702643 > 0 (positive significant variation); green: E-value < 0.05 and t-value < 0 (negative significant variation). (B): Gene expression patterns for the functional categories discussed further in the text. Wild-type cells were treated with selenite and gene expression was evaluated by microarray analyses at different time points, using untreated cells as a reference. Note that the lists of genes represented here are not exhaustive and represent a sample of the genes present in these GO categories. More complete information can be found in additional file 1.Page 3 of(page number not for citation purposes)BMC Genomics 2008, 9:http://www.biomedcentral.com/1471-2164/9/effects on growth, triggered a large ESR [1]. This response was characterized by the general repression of genes involved in cytosolic translation (ribosome biogenesis, rRNA and tRNA processing, etc.) and by the induction of chaperone proteins and genes involved in energy storage and carbohydrate metabolism (figure 1A). This probabilistic model of the selenite response was confirmed by the expression pro.Is factor in coordination of the oxidative stress response.ResultsGene ontology mapping of the selenite response We analyzed the transcriptome of S. cerevisiae cells treated with 1 mM sodium selenite for 2, 5, 10, 20, 40, 60 and 80 minutes.
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